You are interested in a eukaryotic protein involved in immunity, and you are att

Question

You are interested in a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli in order to produce large amounts of the protein. You have identified the gene and place a copy of the gene on a plasmid in E. coli next to a bacterial promoter sequence. You determine that’s lots of mRNA is made from your gene in your E. coli system, but protein produced is larger and doesn’t have the same properties as the eukaryotic protein you expected. What mistakes have you made and how can you fix it? You are interested in a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli in order to produce large amounts of the protein. You have identified the gene and place a copy of the gene on a plasmid in E. coli next to a bacterial promoter sequence. You determine that’s lots of mRNA is made from your gene in your E. coli system, but protein produced is larger and doesn’t have the same properties as the eukaryotic protein you expected. What mistakes have you made and how can you fix it?

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You are interested in a eukaryotic protein involved in immunity, and you are att

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