1. Restriction enzymes (select all that apply): Are necessary for joining two DN
ID: 263793 • Letter: 1
Question
1.
Restriction enzymes (select all that apply):
Are necessary for joining two DNA fragments together.
2.
A polylinker is:
A sequence that allows for selection of cells that contain the vector.
3.
A plasmid vector typically has which of the following features? Select all that apply.
Unique restriction sites for insertion of DNA.
4.
A DNA sequence of interest was digested and ligated into the middle of the AmpR gene region of pBR322 vector. The vector also contains a TetR gene which is not interrupted. After introducing the recombinat DNA into bacterial cells, the resulting transformants were tested on a variety of media. Which of the following results would be expected?
Growth on ampicillin containing media.
5.
How are recombinat DNAs introduced into bacterial cells?
6.
Which of the following is true of genomic and cDNA libraries? Select all that apply.
cDNA libraries contain cDNAs made from all types of RNA.
7.
Overexpression of proteins using bacterial vectors:
Always produce sticky ends.Explanation / Answer
1. Restriction enzymes (select all that apply): Are necessary for joining two DN
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