I\'m currently designing a flow cytometry experiment testing the effect of diffe

Question

I'm currently designing a flow cytometry experiment testing the effect of different concentration of fish oil on PMBC (human blood peripheral cells). I'm allowed to run a 6 color experiments, so what subsets should i use and what do i expect for the results between each subsets and what 6 fluorophores should i use and with which between the available reagents : Annexin-V-FITC, CFSE, Cell-Trace-Violet, Hoescht-3342/DAPI, Mitrotracker-Orange, PI, WGA-AF488 & UV-aqua (live-dead), FITC, APC, ACP-H7, APCY-7, PE, PERCP

The list of markers are: CD3,19, 4,8, HLA-DR, 56, 11, 69

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I'm currently designing a flow cytometry experiment testing the effect of diffe

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