4. The process that some bacteria and lower eukaryotes use that reverses thymine

Question

4. The process that some bacteria and lower eukaryotes use that reverses thymine-thymine dimers in their DNA that has been caused by ultraviolet radiation is called: (1pt) 5. The laboratory narrative for the 'Physical Control' lab listed some reasons that bacteria do not grow below their minimum growth temperature and above their maximum growth temperatun List one of these reasons for each temperature limit.(2 pts) At or below the Minimum Growth Temperature: At or below the Maximum Growth Temperature: 6. List any two of the six factors (given on page 2 of the laboratory narrative) that may affect the zo diameter when using the Disk Diffusion assay to determine antibiotic susceptibility. (2pts) 2.

Explanation / Answer

4. DNA repair. Its a process where the wrong bases are corrected by specific enzymes in this case photolyases

5. At or below minimum temperature: this is because the metabolism slows down at low temperatuer, besides every biological process is enzyme mediated and enzymes have an optimal temperature of working

At or above maximum temperature: this is because at higher temperature, the water evaporates to cause dehydration of the medium, besides the enzymes are degraded at high temperatures

6. the factors that affect zone diameters are:

a) concentration of the antibiotic poured on the disc

b) number of bacteria plated on the agar media

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