6. You are studying a cargo protein which you believe is involved in nuclear tra
ID: 262889 • Letter: 6
Question
6. You are studying a cargo protein which you believe is involved in nuclear transport. You have created various truncation mutations in the cargo protein in which certain portions of are removed. You determine whether the cargo protein localizes to the nucleus and/or cytoplasm under normal conditions and also in the presence of leptomycin B, an inhibitor of the nuclear export machinery Your data are shown in the following figure. (A plus means the cargo protein is located in that part of the cell, a minus that it isn't.) 1-70 451-750 301-430 5-430Explanation / Answer
B. As, cargo protein can pass through the nuclear membrane because of the presence of nuclear transport receptors. Hence, according to the figure, since first protein have a signal for nucleus and cytoplasm and in the presence of inhibitor, too the nucleus receptor is there , followed by second protein.
b. Nuclear localization sequence is thought to be present in 151-450 amino acid as this stretch of sequence is present in all the sequences,.
c. Nuclear export signal have residue 1-150
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