Question 21 (1 point) You are interested in seeing how a large subset of genes a

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Question 21 (1 point)

You are interested in seeing how a large subset of genes are conserved between 2 different species. Which technique might best allow you to investigate this?

Question 21 options:

Dideoxy sequencing

Next-gen sequencing

RNA-seq

Either A or B

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Question 22 (1 point)

Consider an experiment where you want to compare what is happening in a normal cell vs. a cancerous cell. Which technique would allow you to see changes in gene expression between the two different cell types?

Question 22 options:

Dideoxy sequencing

Next-Gen sequencing

RNA-seq

Either A or B

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Question 23 (1 point)

What is the role of Cas9 in the CRISPR-Cas9 genome editing system?

Question 23 options:

It is an enzyme that repairs double-stranded DNA breaks in the genome.

It is an endonuclease that cuts double-stranded DNA at specific sequences.

It is a kinase involved in signaling during DNA repair.

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Question 24 (1 point)

You are a scientist trying to knockout the Sox9 gene in a zebrafish embryo using the CRISPR-Cas9 genome editing system. Upon further analysis you find that you did not knockout Sox9, but instead you created a mutation in a completely different location in the genome. What could be a possible explanation for this unexpected result?

Question 24 options:

After cutting the DNA, the break in the Sox9 gene was fixed perfectly by the DNA repair mechanisms, so no mutation occurred.

Your Cas9 was not functioning correctly.

Your guide RNA wasn't targeting the right sequence in the genome.

You can't perform genome editing in the zebrafish genome.

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Question 25 (1 point)

The cell cycle is tightly regulated by:

Question 25 options:

the presence of certain growth factors.

cyclins.

cyclin-dependent kinases.

all of the above are involved in cell cycle regulation

Dideoxy sequencing

Next-gen sequencing

RNA-seq

Either A or B

Explanation / Answer

21. Option B is correct.

Next-gen sequencing is used to study a large set of genes.

22. Option C is correct.

Changes in gene expression can be studied by RNA-seq.

23. Option B is correct.

Cas9 is an endonuclease that cuts DNA at specific locations.

24. Option C is correct.

The target specificity in CRISPR-Cas system is achieved by guide RNA sequence complementarity.

25. Option D is correct.

The cell cycle is regulated by growth factors, cyclins, and cdks.

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