How can you tell whether the expression of this gene is under transcriptional or

Question

How can you tell whether the expression of this gene is under transcriptional or translational control? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Terms can be used once, more than once, or not at all Reset Help RNA DNA mRNA hnRNA northern blotting western blotting southern blotting transcriptional An approach to determine the level of control of a particular gene is to identify whether levels of are consistent among various cell types of interest. This is often accomplished by or by use of in situ hybridization either direct isolation of the RNA and assessment by If the control can be eliminated as a possibility Support for translational control can be achieved directly by determining, in different cell types, the presence of a variety of only when sufficient knowledge exists for specific pools for a given gene are consistent in various cell types, then species with common sequences. This can be accomplished trapping or labeling Clues as to translational control via alternative splicing can sometimes be achieved by examining the translational acid sequence of proteins. Similarities in certain structural/functional motifs may amino indicate alternative processing nucleic

Explanation / Answer

An approach to determine the level of control of a particular gene is to identify whether levels of mRNA are consistent among various cell types of interest. This is often accomplished by either direct isolation of the RNA and assessment by Northern blotting or by use of in situ hybridization. If the RNA pools for the given gene are consistent in various cell types, then transcriptional control can be eliminated as a possibility.

Support for translational control can be achieved directly by determining, in different cell types, the presence of a variety of DNA species with common sequences. This can be accomplished only when sufficient knowledge exists for specific nucleic trapping or labeling.

Clues as to translational control via alternative splicing can sometimes be achieved by examining the amino acid sequence of proteins. Similarities in certain structural/functional motifs may indicate alternative translational     processing.

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