A procedure called chromatin immunoprecipitation allows scientists to determine

Question

A procedure called chromatin immunoprecipitation allows scientists to determine where a particular protein is located in the genome. If you performed chromatin immunoprecipitation on acetylated histones, where in the genome would you expect to find these modified histones? A procedure called chromatin immunoprecipitation allows scientists to determine where a particular protein is located in the genome. If you performed chromatin immunoprecipitation on acetylated histones, where in the genome would you expect to find these modified histones?

Explanation / Answer

Chromatin Immunoprecipitation is a advance and dynamic technique for the detection and analysis of protein DNA interaction.The physiological interaction and attachment of protein with chromosomal DNA is studied, using the concept behind Antigen and antobody ineteraction.The status of Histone binding with DNA directly or indirectly are analysed and mapped using microplate based technique called ChlP.Histone DNA binding is significant for proper and systematci cellular mechnisms like chromosomal segregation,gene transduction ,.transcription ,DNA replication and recombination.

Histone modification happense at post translational modification stage of DNA replication process.The modification includes methylation,acetylation ,phosphorylation,sumoylation and ubiquitylation.Such modufcation effect the normal function of chromatin by changing the structure of chromatin hence cause gene expression mutation.

Histone acetylation is linked with the open chromatin structurewhich ssist in gene exptression ,activation or silencing of transcription,apoptosis and cell division.Histone acetylaion is a enzymatic procedure in which the acetyle group is added in the presenc eof acetyle co enzyme.The enzyme take part in the Histone modification is Histine acetyle transferase(HATs) which can also regulate the acetylation of H4 and H3.

Histone acetylation is found mostly in H3 at the amino acid site where lysines K9,K14,K18,K23 ,K56 and H4 at lysines K5,K8,K12 and K16.This can be highly visible in cacer and at various pathological condition.

eg-Th epresence of H4 acetylation at K12 is detected with the help pf the strip which is coated with H4 K12 antibody and then followed with flourosence development.The presence of acetylation depends on the intensity of flourscence absorbtion which is quantified by comparing with standard.

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