Suppose, you synthesized RNA in vitro using polynucleotide phosphorylase, 3 part

Question

Suppose, you synthesized RNA in vitro using polynucleotide phosphorylase, 3 parts ADP and one part GDP. After the reaction, you isolated all RNA molecules and translated them in a cell-free translation system.

Now, answer the following questions.

(A) How many different amino acids do you expect in the polypeptides synthesized in the cell-free system? Name these amino acids. Use the codon dictionary available in the textbook.

(B) Which amino acid would show highest frequency of incorporation in the polypeptides synthesized in the cell-free system? Show your calculations for full credit.

Explanation / Answer

1. 8 codons are coded by A and G and that 8 codons will code only 4 amino acids

AAA, AAG both code for lysin

AGA, AGG both code for arginin

GAA, GAG both for glutamic acid

GGA, GGG both for glycin

2. A:G = 3:1 so A incorporate 3times then G

so chances of AAA, AAG, AGA, GAA are more so lysine will be coded in high frequency

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