2. Case: Senior smoker succumbs to severe spasms A 65-year old man comes into yo
ID: 261289 • Letter: 2
Question
2. Case: Senior smoker succumbs to severe spasms A 65-year old man comes into your clinic with labored breathing and complains that his chest hurts. He presents with fever, chills, and a spasmodic cough. He is coughing up green sputum and a chest X-ray reveals fluid in his lungs. He has been a 2 pack/day smoker for the past forty years and also has diabetes. You order a blood culture/Gram stain to determine what is causing his symptoms You are given a blood agar plate and a Gram stain of his culture. Record your obscrvations below: Blood agar shows Gram reaction Cell morphology Cell arrangement You would like to do a catalase test on the isolated pathogen. What reagent will yoa need in order to do this test? The catalase test comes back negative. What would this look like? The following tubes were inoculated with the isolated colonies: Bile esculin and BHI with 6.5% NaCl Both gave negative results. The culture was also tested for antibiotic sensitivity and the following results were obtained:Explanation / Answer
Blood agar shows alpha hemolysis
Hydrogen peroxide is required to perform catalase test
No bubbles were observed in negative sample
This tells that the person is suffering from pneumoniae, the isolated organism is a gram positive bacteria
The organism responsible for his illness is Streptococcus pneumoniae
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