on. bp bp Enzyme 1 (cuts @ 1000 bp) Enzyme 2 (cuts a 5500 bp) bla (aka AmpR) Enz

Question

on. bp bp Enzyme 1 (cuts @ 1000 bp) Enzyme 2 (cuts a 5500 bp) bla (aka AmpR) Enzyme 3 (cuts @ 4200 bp) Lac Z Enzyme 3 (cuts @ 1700 bp) bla (aka Amp) Enzyme l (cuts a 2000 Enzyme 2 (cuts @2100 bp) Plasmid A-6000 bp Plasmid 'B'-6000 bp A student was given a transformed E. coli culture. The transforming plasmid was one of the two shown above. They have isolated the plasmid for use in molecular analyses. 11) A streak plate was made using nutrient medium supplemented with ampicillin. Colonies that grew on this medium where white in color. Based on this line of evidence, which plasmid appears to be the transforming plasmid? a) Plasmid 'A b) Plasmid 'B c) could be either 'A' or 'B' d) neither 'A' nor 'B" -12) A streak plate was made using nutrient medium supplemented with ampicillin and X-gal. Th colonies that grew on this medium where white in color. Based on this line of evidence, which plasmid appears to be the transforming plasmid? a) Plasmid 'A b) Plasmid 'B' c) could be either 'A' or 'B' d) neither 'A' nor 'B' 13) A streak plate was made using nutrient medium supplemented with ampicillin and X-gal. Colonies that grew on this medium where blue in color. Based on this line of evidence which plasmid appears to be the transforming plasmid? a) Plasmid 'A b) Plasmid 'B' c) could be either ‘A' or ‘B' d) neither 'A' nor 'B'

Explanation / Answer

Question 11- Both plasmids have Ampicillin resistant gene. Therefore, cells containing any of the two plasmids will survive under ampicillin. Therefore, the correct option is 3rd option ‘c’.

Question 12- in this medium we have Ampcillin with X-gal that is usable by beta galactosidase enzyme. The enzyme produces by Lac Z that is present only in one plasmid. If such plasmid was in the culture, then X-gal must convert into an indigo or blue colored product. However, we get white colored colony in this culture. Therefore, the right answer is Plasmid B, which is option 2nd (b).

Question 13- Here we got blue colored colony. Therefore, the colonies must contain Plasmid A. Therefore, the correct option is the first option (a).

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