Practice Question Hematopoietic stem cells (HSC) reside in the bone marrow and g

Question

Practice Question Hematopoietic stem cells (HSC) reside in the bone marrow and give rise to all of the cells in the blood. When not needed, the cells are quiescent (non-active), but when more blood cells are needed, signals arrive that activate the HSC to enter the cell cycle to make more blood cells. We wanted to identify genes that might regulate quiescence of HSC so we performed RT-PCR for HIF-1(hypoxia inducible factor-1) and HIF-2 on quiescent HSC (QHSC) and activated HSC (aHSC) and got the results seen on the gel. Lane 1- HIF-1 on gHSC, Lane 2- HIF-1 on aHSC, Lane 3- HIF-2 on aHSC, Lane 4-HIF-2 on aHSC, Lane 5= ?-actin on aHSC, and Lane 6: ?. actin on aHSC. Each set of primers give the same size band. A: (2 pts) What did we isolate from the HSCs in order to do this experiment? B: (3 pts) What seems to be happening to HIF-1 expression? .C: (3 pts) What seems to be happening to HIF-2 expression? .D: (2 pts) Do you think either of these genes may play a role in HSC quiescence and if so which one?

Explanation / Answer

A: mRNA  

B: HIF-1 decreases upon activation of the HSC

C: HIF2- expression remians constanst whatsoever.

D. HIF-1

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