4. Three different eukaryotic RNA polymerases are required to synthesize messeng

Question

4. Three different eukaryotic RNA polymerases are required to synthesize messenger RNA molecules, and translate them into proteins.

a. Which type of eukaryotic RNA polymerase is required to synthesize the transcripts that will become messenger RNA molecules?

b. Which type of eukaryotic RNA polymerase is required to make the small nuclear RNA molecules required for splicing?

c. Which two types of eukaryotic RNA polymerase is required to make the ribosomal RNA components of the ribosome.

d. Which type of eukaryotic RNA polymerase is required to transcribe genes encoding ribosomal protein molecules.

e. Which type of eukaryotic RNA polymerase is required to transcribe transfer RNA molecules?

f. Where specifically in the cell are the ribosomal components assembled to form the small and large ribosome subunits?

g. Functional bacterial ribosomes can be self-assembles from purified RNA and protein components. However, a similar attempt to self-assembled a functional eukaryotic ribosome fails. What additional components are required to form a functional ribosome in eukaryotes?

h. Transfer RNA and ribosomal RNA molecules contain bases other than A, C, G, and U. How is this possible?

Explanation / Answer

A) RNA Polymerase-II is required to synthesize the transcripts that will become messenger RNA molecules.

B) RNA polymerase II is required to make the small nuclear RNA molecules required for splicing.

C) RNA polymerase I and III are required to make the ribosomal RNA components of the ribosome.

D) RNA polymerase I is required to transcribe genes encoding ribosomal protein molecules.

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