Quiz 11:ONA and Biotechnology blood Bob Sue John Lis Questions 1-8 are worth 1 p
ID: 260740 • Letter: Q
Question
Quiz 11:ONA and Biotechnology blood Bob Sue John Lis Questions 1-8 are worth 1 point each. For question 1-3 below, use the diagram of a gel electrophoresis of DNA samples from several individuals (one sample per lane of the gel) shown to the right found at a crime scene. 1. Which individual could have been the person whose blood made the blood stain at the crime scene? a. Sue b. John c. Lisa eob of therm e. None of them 2, why did you choose the individual from question #1? a. The DNA fingerprints match b. The individual had the same number of bands, but in different locations on the gel. c. Only the blood stain and the individual had RFLPs in their samples. d. There was no match. 3. Restriction enzymes, found in cells, have an original function in the cell. However, molecular biologists have learned this function and now use it in many laboratory settings, including solving crimes involving murder. What is the original function of a restriction enzyme in a cell? a. To degrade RNA into nucleotides by breaking hydrogen bonds b. To begin the process of Transcription and Translation c. To restrict the invasion of foreign DNA by cutting it up into tiny pieces d. To lyse white blood cells In what orientation can the enzyme DNA polymerase add a DNA monomer to an existing DNA molecule? 4. a. Added to the 3' carbon b. Added to the 5'carbor c. Added to the nitrogenous base d. Added to the phosphate groupExplanation / Answer
Ans 1- option B John
The DNA fingerprinting pattern of sue, Lisa and Bob has 7, 7 and 8 RFLP bands respectively but the sample obtained from crime scene has 9 RFLP bands and the position and pattern of these bands exactly matches with the band no. and pattern of John.
Ans 2- option A (DNA fingerprints matches)
DNA fingerprinting technique depends on the RFLP fragments generated by restriction enzymes. If the nucleotide Composition is same, same pattern of DNA fingerprints are generated as in this case.
Ans 3- option C (To restrict the invasion of foreign DNA by cutting it up into tiny pieces)
RNAse are the enzymes which breaks hydrogen bonds in nucleotide.hence option A is incorrect
Transcription is done by polymerase enzymes while translation involves many enzymes other than restriction endonuclease hence option B is also incorrect.
To Lyse WBCs haemolytic enzymes are responsible and not restriction enzymes hence option D is also incorrect.
Restriction endonuclease are enzymes produced by bacteria to protect themselves from foreign DNA of bacteriophage.
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