Primer design is a critical step for the detection of a particular sequence in a

Question

Primer design is a critical step for the detection of a particular sequence in a sample. Which of the following would be true of primer design?

A. RNA polymerase are added to the PCR mix to design random primers B. If detecting viral infection by PCR, the primers must not be complimentary (match) to regions of human DNA C. Pairs of primers must be designed, one to start amplification with Taq polymerase, the other to start amplification with human polymerase D. Primers are optional if you want to amplify all DNA within a sample

Explanation / Answer

The answer is - B. If detecting viral infection by PCR, the primers must be complimentary to regions of human DNA.

Primers are complimentary sequences of small size used to amplify DNA by PCR. And in PCR reaction human polymerase is not used. And primers are necessary for DNA replication through PCR because no polymerase can synthesize DNA with out primers. Because they need free 3' OH group.

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