You notice that transfection of this cDNA in mammalian CHO cells results in tran

Question

You notice that transfection of this cDNA in mammalian CHO cells results in transfected cells with a distinct shape. You are considering whether the transfected cDNA might affect the function of actin, so you review how actin polymerizes.

For the following question, you may choose more than one answer.

Which of the following are true of actin polymerization? Provide a n explanation for your answers.

A. If the cytosolic concentration of G-actin is above both the Cc+ and Cc- , this results in addition at both ends of the actin filament.

B. If the cytosolic concentration of G-actin is above both the Cc+ and Cc- , this results in addition only on the positive end of the actin filament.

C. If the cytosolic concentration of G-actin is below both the Cc+ and Cc- , this results in addition at both ends of the actin filament.

D. If the cytosolic concentration of G-actin is below both the Cc+ and Cc- , this results in subtraction at both ends of the actin filament.


Also, provide a description of treadmilling.

Explanation / Answer

B.If cytosolic concentration of G actin is above both the Cc+ and Cc- this results in the addition on the the positive end of the actin filament.Cc here stands for the critical concentration which is needed for polymerisation into the F actin.The length of the filament remains constant the number of subunit added to the positive end and no. Of subunits are removed from the minus end.

D.If the cytosolic concentration of G actin is below the Cc+ and Cc- this results in the subtraction at both ends of the actin filament.A critical concentration is always required to maintain for the addition of G actin.So it will subtraction of actin from the filament.

Treadmilling: It occurs when one side of the filament grows in length while the other ends shrink due to removal of subunits from the minus end.Actin filament is a very dynamic structure.Protein subunits are being added at the one end while protein subunit are being removed at one end.

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