9. Eukaryotic gene expression Are these all of the cis sequences necessary for W
ID: 259969 • Letter: 9
Question
9. Eukaryotic gene expression
Are these all of the cis sequences necessary for WT pigment expression patterns? (YES/NO)
Are the three regulatory regions upstream or downstream of the gene?
Explain what regulatory element is present (if any) in regions 1-3.
region 1_______________________________________________________________
region 2_______________________________________________________________
region 3_______________________________________________________________
D. Read the description of the experiment carefully. What is the mutation in the GW mutant? Explain whether it is a protein or a cis sequence, which cells are affected (whole body, top wing, white patch, etc) and how the mutation causes the GW pattern.
WT GW mutants 123P reporter P reporter 2H Preporter P reporter P reporter WT pattern Greywing mutant pattern 3 P reporter Reporter gene expression in WT and mutant insectsExplanation / Answer
1) All the gens CIS genes are required to have the desired wild type strains as we can see in the diagram that missing of any genes have caused a different phenotype which are mutant strains and presence of three gens 1,2,3 have produced wild type phenotype by this we can say that all the three gens are required for the reporter.
2) The regulatory sequences are downstream as the promoter is present after the genes and it should all along the gens in the downstream to synthesize the protein for the phenotype.
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