Butterflies again! These butterflies have only one pigment gene; dark areas have
ID: 259916 • Letter: B
Question
Butterflies again! These butterflies have only one pigment gene; dark areas have high levels of expression, grey areas have medium levels of expression, and white areas have no pigment expression. You've also isolated a butterfly (Greywing) that has a mutation- the white patches are missing. To determine the regulation of this gene's expression, you construct reporter genes with the promoter (P) and some potential regulatory regions (1-3) from the WT butterfly genome. You insert the six reporter genes into the genomes of WT and Greywing mutants and analyze reporter gene expression. (Note: promoter alone is insufficient to drive reporter gene.)
1. Are these all of the cis sequences necessary for WT pigment expression patterns? (YES/NO)
2. Are the three regulatory regions upstream or downstream of the gene?
WT GW mutants 1H2H3H P reporter 1P reporter P reporter P reporter 12-P reporter 2 3 P reporter 2 WT patterrn mutant pattern Reporter gene expression in WT and mutant insectsExplanation / Answer
1) All the gens CIS genes are required to have the desired wild type strains as we can see in the diagram that missing of any genes have caused a different phenotype which are mutant strains and presence of three gens 1,2,3 have produced wild type phenotype by this we can say that all the three gens are required for the reporter.
2) The regulatory sequences are downstream as the promoter is present after the genes and it should all along the gens in the downstream to synthesize the protein for the phenotype.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.