During the blood typing studies, the reaction consisted of 2 drops of antiserum

Question

During the blood typing studies, the reaction consisted of 2 drops of antiserum plus 1 drop of red blood cells. How would the results be affected if 4 drops of antiserum were used?

no effect – the results would be exactly the same as using 2 drops of antiserum

excess antibody would produce a stronger agglutination of the red blood cells

excess antibody would prevent agglutination of the red blood cells

no effect – the results would be exactly the same as using 2 drops of antiserum

excess antibody would produce a stronger agglutination of the red blood cells

excess antibody would prevent agglutination of the red blood cells

Explanation / Answer

Answer:

Answer is "A" - no effect, the results would be exactly the same as using 2 drops of antiserum.

Note: In this reaction, antibodies are bound with antigens at specific sites already at a perfect ratio. hence there could be no more binding sites for antibodies to attach. so the reaction will be same.

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