3. How does separation of DNA fragments via electrophoresis differ from separati

Question

3. How does separation of DNA fragments via electrophoresis differ from separation of Proteins? We use Polyacrylamide gel for proteins insted of agarosegel that is ured for DNA 4. Answer the following questions about a western blot: a) What is a primary antibody? In our lab, what did it bind to? V The antibody that binds to a protein. yantibody? In our lab, what was attached to the secondary antibody b) What is a secondary antibody? In our lab, what was at so we could detect it? The secondary antibody attaches to the primar which then makes it detectable oecond hos lizymy

Explanation / Answer

Secondary antibody is an antibody that attaches to the primary antibody in order to ensure that the primary body is bound with antigen and secondary antibody is enzyme linked and appropriate substrate is used for the particular enzyme that has been attached to the secondary antibody to detect the conjugation of primary and secondary antibody.

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