Below is the DNA sequence that codes for a normal hemoglobin protein. Using the

Question

Below is the DNA sequence that codes for a normal hemoglobin protein. Using the DNA sequence provided in the 3' to 5' direction, transcribe the sequence into mRNA. 1) 3'TGACGGGTACCACGTGGACTGAGGACTCCTC5 2) For normal hemoglobin, translate the mRNA strand from question 1 to the correct amino acid sequence. Make sure to start translation only after reaching the start codon Below is the DNA sequence that codes for a sickle cell hemoglobin protein. Using the DNA sequence provided in the 3' to 5' direction, transcribe the sequence into mRNA. 3) 3'TGACGGGTACCACGTGGACTGAGGACACCTC5' For sickle cell hemoglobin, translate the mRNA strand from question 3 to the correct amino acid sequence. N sure to start translation only after reaching the start codon. 4)

Explanation / Answer

1)

3' TGACGGGTACCACGTGGACTGAGGACTCCTC 5'

5' ACTGCCCATGGTGCACCTGACTCCTGAGGAG 3' Sense strand of DNA

5' ACUGCCCAUGGUGCACCUGACUCCUGAGGAG 3' mRNA strand

2)

5' AUG(start codon) GUG CAC CUG ACU CCU GAG GAG 3'

Amino acid sequence : Met Val His Ieu Thr Pro Glu Glu

3)

3' TGACGGGTACCACGTGGACTGAGGACACCTC 5'

5' ACTGCCCATGGTGCACCTGACTCCTGTGGAG 3' Sense strand of DNA

5' ACUGCCCAUGGUGCACCUGACUCCUGUGGAG 3' mRNA strand

4)

5' AUG(start codon) GUG CAC CUG ACU CCU GUG GAG 3'

Amino acid sequence : Met Val His Ieu Thr Pro Val Glu

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