Questions based on the following article: MAP kinase phosphorylation of plant pr
ID: 258679 • Letter: Q
Question
Questions based on the following article: MAP kinase phosphorylation of plant profilin Apinun Limmongkona,1, Concetta Giuliania,1, Rudolf Valentab , Irene Mittermannb , Erwin Heberle-Borsa , Cathal Wilsona,c,*
Abstract: Did the abstract state the overall research question, brief materials and methods used to address question, describe results and relate findings to field?
Introduction: State 3 specific background facts found in the introduction. What do the authors suggest is the major accomplishment(s) of the manuscript?
Results/Figures: Which figures/tables address the major research question(s)? Did the results directly address the research question(s)? Were the figures presented in a logical manner?
Conclusions: Do the conclusions/discussions relate to the major question(s). Does the data support these conclusions, why or why not?
Explanation / Answer
Introduction :
Profilin is a small actin-binding protein and is expressed at high levels in mature pollen where it is thought to regulate actin filament dynamics upon pollen germination and tube growth.
The majority of identified plant profilins contain a MAP kinase phosphorylation motif, P-X-T-P, and a MAP kinase interaction motif (KIM).
In in vitro kinase assays, the tobacco MAP kinases p45(Ntf4) and SIPK, when activated by the tobacco MAP kinase kinase NtMEK2, can phosphorylate the tobacco profilin NtProf2.
Result :
Mutagenesis of the threonine residue in this motif identified it as the site of MAP kinase phosphorylation.
Conclusion :
Fractionation of tobacco pollen extracts showed that p45(Ntf4) is found exclusively in the high-speed pellet fraction while SIPK and profilin are predominantly cytosolic.
These data identify one of the first substrates to be directly phosphorylated by MAP kinases in plants.
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