I need answers to these two questions: - In the murine kappa Ig light chain DNA,

Question

I need answers to these two questions:

- In the murine kappa Ig light chain DNA, there is a BamH1 restriction enzyme site. Where on the DNA 3’-5’ strand is this restriction enzyme site relative to both the variable and constant coding region (3’ or 5’?)? Is the restriction enzyme site always there? How do you know (what data/evidence support) a) where the restriction enzyme site is and b) if the restriction enzyme site is there?

-Describe evidence that Rag-1 and Rag-2 expression are sufficient to initiate Ig gene rearrangement and without them, you can’t get Ig gene rearrangement?

Explanation / Answer

The restriction enzyme will not be always present. This enzyme is produced by certain bacteria that has the property of cleaving DNA molecules near a specific sequensv of bases.

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