1. Why is permount necessary to use in coverslipping for Nissl- stained tissue?
ID: 258362 • Letter: 1
Question
1. Why is permount necessary to use in coverslipping for Nissl- stained tissue?2. Why isn’t permount used for DAPI stained tissue?
3. Why should the DAPI tissue be covered after DAPI staining? 1. Why is permount necessary to use in coverslipping for Nissl- stained tissue?
2. Why isn’t permount used for DAPI stained tissue?
3. Why should the DAPI tissue be covered after DAPI staining?
2. Why isn’t permount used for DAPI stained tissue?
3. Why should the DAPI tissue be covered after DAPI staining?
Explanation / Answer
Please find the answers below:
Answer 1: Nissl-stain is an organi-acid based stain which gives bright blue and pink color to the tissue. Thus, in order to permanently seal the tissue-slides stained with Nissl-stain, the slides/coverslips are often mounted in permount as a medium of choice.
Answer 2: Since permount is a stain which permanently seals the glass slide but cannot prevent photo-bleaching of fluorescent dyes, it is not mandatory to mount a DAPI-stained tissue in permount. On the contrary, permanents mounting media are commercially available which prevent photo-bleaching.
Answer 3: Since DAPI is a photo-sensitive stain/dye, it can undergo rapid photo-bleaching and thus loss of signal due to exposure to light. Hence, the DAPI-stained tissues are always covered after performing the staining proces.
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