The image of the gel below shows the bands resulting from a Sal I digestion of p
ID: 258056 • Letter: T
Question
The image of the gel below shows the bands resulting from a Sal I digestion of pCamR-GFP and a Sal I digestion of pUC18-207 in either lane 2 or 3 (part of the question requires you to determine which digestion was loaded into which lane). Which of the restriction fragments shown below correctly corresponds to the red band indicated by the asterisk (*)? (2 marks, all or none)
Question 5 Answer saved Marked out of 2.00 P Flag question The image of the gel below shows the bands resulting from a Sal I digestion of pCam*-GFP and a Sal I digestion of pUC18-207 in either lane 2 or 3 (part of the question requires you to determine which digestion was loaded into which lane). Which of the restriction fragments shown below correctly corresponds to the red band indicated by the asterisk (*)? (2 marks, all or none) 1 kb ladder Lanes 8000 bp 7000 bp 6000 bp 5000 bp 4000 bp 3000 bp 2000 bp 1000 bp 100 bp 2 Select one: Not l Bam HI O A. SalI Bam H ac Pst l Eco RI Bam Hl Sal I Hind Ill GFP CamR ORI cross out B. SacNotXba Sal I Bsa I Pfl1081 AlWN Afl Ili Sap Amp ORI promoter MCSExplanation / Answer
Answer:
Lane 2 contains the Sal I digestion of p-camR-GFP digestion and lane 3 contains the pUC18-207 sal I digestion. Both of these are double digested with Sal I producing two bands of different lengths. The ultimate aim of the researcher is to clone the CAMR GFP gene into pUC18-207 vector.
Therefore the band with asterics mark should contain a terminal GFP portion along with camR segment. Therefore the restriction fragment (A) looks like the correct restriction fragment.
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