Each multiple choice/fill in the blank is worth 2 points. 1. The term proteomics

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Each multiple choice/fill in the blank is worth 2 points. 1. The term proteomics refers to a Global Analysis of the proteins produced in an organism b. All of the mRNA transcribed at a given time. c. The genes that are expressed at a given time. d. None of the above. 2. Techniques used to separate and study proteins include all of the following EXCEPT a. Isoelectric focusing b. Agarose gel electrophoresis c. Polyacrylamide gel electrophoresis d. Two-dimensional PAGE e. All of the above can be used to separate and study proteins 3. In order to separate proteins by size, one should a. Run acrylamide electrophoresis on crude extracts b. Run agarose gel electrophoresis on crude extracts. c. Run cleavage reactions to remove all acetyl phosphate, AMP or ADP-ribosyl groups prior to gel electrophoresis. Boil with sodium dodecyl sulfate to unfold and provide a uniform negative charge on the protein prior to gel electrophoresis. Proteins cannot be separated by size; they must be separated by charge. d. e. Scientists will identify specific proteins using western blots. Which of the following is the correct order of steps in performing this technique? 4. a. Proteins are reacted with antibody, PAGE is run, proteins are blotted to nitrocellalose and the b. PAGE is run, proteins are blotted to nitrocellulose, then primary antibody is added followed by a c. PAGE is run; primary antibody is added, followed by a secondary antibody with enzyme reporter. second antibody with enzyme reporter is applied secondary antibody with enzyme reporter PAGE is run, proteins are blotted to nitrocellulose, and an antibody with a reporter enayme is then d. added 5. MALDI is a mass spectrometry technique that allows very large molecules such as proteins to be analyzed The best description of MALDI is: a. Liquid samples containing proteins are adhered to a metal bar. The protein is ionized and the los b. Proteins are dissolved in liqui c. Proteins are embedded into 2-methoxycinnamic acid. Laser energy then causes proteins to lose d. None of the above describe MALDI. detected by TOF and ions are released into the vacuum tube and detected by TOF ions, move through a vacuum, and be detected by TOF d, which is turned into droplets. After heating, the solvent evaporates In which of the following ways can mass spectrometry be used to determine the sequence of large proteases? 6. Protein in extracts are digested with proteases and then separated by SDS-PAGE. Individual peptides are sequenced by mass spectrometry and then the sequence is pieced back together. a.

Explanation / Answer

ANSWER 1.
a. Global Analysis of the proteins produced in an organism

ANSWER 2.
e. All of the above can be used to separate and study proteins

ANSWER 3.
d. Boil with sodium dodecyl sulfate to unfold and provide a uniform negative charge on the protein prior to gel electrophoresis.

ANSWER 4.
b. PAGE is run, proteins are blotted to nitrocellulose, then primary antibody is added followed by a, secondary antibody with enzyme reporter.

ANSWER 5.
a. Liquid samples containing proteins are adhered to a metal bar. The protein is inoized and the ions detected by TOF.

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