ou toey 2. A group of researchers at the university veterinary agnostics laborat
ID: 257127 • Letter: O
Question
ou toey 2. A group of researchers at the university veterinary agnostics laboratory isolated a new bacterium from the blood and stool of several horses that became ill and died at a local farm. Prior to death, symptoms included disorientation, loss of motor function, and flaccid paralysis, so the researchers suspected central nervous system involvement and possible production of neurotoxin. Based on 16S rRNA comparison, they found that the new bacterium was related to the gram-positive bacterium Clostridium botulinum, and they named it Clostridium equiniae. The researchers subsequently developed a mouse model of infection, in which bacteria are injected into the bloodstreams of mice, and the mice are monitored for paralysis and death. Using this animal model, they have conducted signature-tagged mutagenesis to identify genes in-Explanation / Answer
The 50 % lethal dose (LD50) - control without antibiotic treatment to be 10, the metronidazole treatment alone to be 104 and the combination of azithromycin and metronidazole treatment to be 107. The experiment reveals that the progression and the neurotoxin release from the clostridium equiniae were controlled by the metronidazole treatment alone, yet the bacterial population raised against the drug with resistant capability. Whereas the combination of azithromycin and metronidazole treatment seems to completely kill the clostridium equiniae population regardless of its resistance and prevent its neurotoxin release which affects the central nervous system.
Experiment: Clostridium culture in three Petri dishes.
1. One Petri dish with no antibiotics treatment (Control)
2. Second Petri dish with metronidazole treatment (T1)
3. Third Petri dish with metronidazole and azithromycin treatment (T2)
All these Petri dishes were cultured with same conditions such as temperature, nutrients, and incubation etc.
Finally, the lethal dosage (LD50) is predicted and experiment data is analyzed determining the dose fixing to kill these bacterial cells in in-Vivo.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.