2. Here is the beginning of a short mRNA transcript that includes through to the

Question

2. Here is the beginning of a short mRNA transcript that includes through to the beginning of exon 2; it has not been spliced yet. >GBYX01165823.1 TSA: Poeciliopsis prolifica 5 AAAUACAAGACUCCAUGGACGGCCAUUUCAGCCCUGGAGUCGACGACACCCGUCACUUUAGAUCGGGGAG3 a) Identify the +1 transcription start site, 5'UTR, exon1, exon2, intron, and splicing signals. b) What is the sequence of the double stranded DNA molecule that produced this transcript? On your DNA molecule, identify, as above, the 1 transcription start site, 5'UTR, exon1, exon2, intron, and splicing signals. c) Splice the above transcript and translate into a polypeptide.

Explanation / Answer

Please find the answers below:

Answer a: The presence of codon AUG or ATG represents the +1 start site on the gene.

The 5' UTR is represented by 5' AGGAGGU site.

The exons present in the sequence are: 5' AUG GAC GGC CAU UUC AGC CCU GGA 3' and 5' AAC UUU AGA UGG GGA 3'

The introns present is:  5' GAC GCA CCC 3'

The splice signals are 5' GU 3' in between the two exons downstream the signal.

Answer b: Since transcription takes place in complementary mode, the parent DNA molecule which would have generated this transcript would be given as below:

5' 5’ AAA UAC AAG ACU CCA GAC GGC CAU UUC AGC CCU GGA GUC GAC GCA CCC GU AAC UUU AGA UGG GGA G 3’

3' (upstream regulator) UUU AUG (start) UUC UGA GGU CUG CCG GUA AAG UCG GGA CCU CA (splice) G CUG CGU GGG CA (splice) UUG AAA UCU ACC CCU C 5'

Answer c: The final mRNA post-transcriptional modification that would be obtained is as below:

5' AUG GAC GGC CAU UUC AGC CCU GGA AAC UUU AGA UGG GGA 3'

The amino acid polypeptide synthesized would be as below:

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