29. Which of the following methods methods circumvents the need for thermal cycl

Question

29. Which of the following methods methods circumvents the need for thermal cycling? A. End-point PCR B. Isothermal PCR C. Real-time PCR D. Reverse transcriptase PCR (RT-PCR) E. All of the above 30. Which of the following methods is used to quantify the amount of the original target sequence? A. End-point PCR B. Isothermal PCR C. Real-time PCR D. Reverse transcriptase PCR (RT-PCR) E. All of the above 31. Which of the following component is NOT required for the Sanger DNA sequencing method? A. Deoxynucleotides (dNTPs) B. Dideoxynucleotides (ddNTPs) C. DNA Primers D. RNA polymerase E. Template DNA 32. Which of the following is NOT true of the Sanger method? A. Also referred to as the chain-termination DNA sequencing method B. Frederick Sanger developed the method C. Most commonly used methood D. Use adapters added to the end of DNA fragments E. All of the above are true

Explanation / Answer

29. B. In isothermal PCR we can bypss the thermal cycling because isothermal PCR is helicase dependent.

30.C. In real time PCR we can identify the original target DNA.

31.D. RNA polymerase is not required for Sanger sequencing method.

32.D. Adapters are not used in Sanger sequencing.

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