You have identified an enzyme from a thermophilic bacterium that lives in hot sp

Question

You have identified an enzyme from a thermophilic bacterium that lives in hot springs which converts glucose into a compound that effectively treats male pattern baldness. When you express your enzyme in E. Coli for mass production of your compound, you do not recover any compound from protein expressing bacterial culture grown in standard laboratory conditions at 37C.

1) Hoping to create a variant with higher activity in E. Coli, you perform error prone PCR to introduce random mutations into the gene. You isolate a more active variant, and determine that it has a very similar molecular eright but a much lower extinction coefficient at 280 nm. Explain why this variant might be a more active E. Coli.

Explanation / Answer

The information provides evidences that the variant is a more active form of E. coli as comparec to wild type variety. To enlist these evidences, the description can be found as below:

Thus, these set of evidences ensure that the variant is a more active form of microbe as compared to the wild type.

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