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QUESTION 8 You have created a cell deficient (knocked out) for gene A. When usin

ID: 256852 • Letter: Q

Question

QUESTION 8 You have created a cell deficient (knocked out) for gene A. When using western blot analysis to characterize the effects of this knockout on the total protein levels and phosphorylation state of protein B, you obtain the following data. Which of the following might you predict to be the function for the protein encoded by gene A? WT Gene A knockout phosphoprotein- specific antibodies protein B protein B protein-specific antibodies O a. Gene A might be a transcription factor that represses the transcription of Gene B. b, Gene A might be a phosphatase that targets Gene B. C. Gene A might be a kinase that targets Gene B d.Gene A might be a transcription factor that activates the transcription of Gene B

Explanation / Answer

Ans b) Gene A might be a phosphatase that targets Gene B. This impacts the phosphorylation of the Gene B and due to the protein produced by the gene A, it leads to the phosphorylation of the Gene B

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