Multiple (3 each) 1- In biotechnology, the purpose of restriction enzymes is to

Question

Multiple (3 each) 1- In biotechnology, the purpose of restriction enzymes is to A. Protect the host DNA from foreign (inserted) DNA B. Cut the DNA fragment (gene) of interest. C inserts the DNA of interest into the host DNA D. Ligase the new DNA in the host DNA 2-Blunt ends are the result of A) B) treatment of a nucleotide sequence with DNA ligase. C) exposure of eukaryotic DNA to a prokaryotic plasmid. D) infection of a cell with a bacteriophage. E) DNA breaking down in the presence of reverse transcriptase. 3-Using genetic engineering genes can be A) moved from one organism to potentially any other organism. B) easily converted to produce any product we desire. C) turned off or on at our whim. D) used to cure all known diseases. E) create plants resistant to all insect pests. 4-DNA are cut in specific location with A) restriction enzymes B) ligases. C) reverse transcriptase. D) polymerase E) plasmids 5-In double-stranded DNA, which of the following base ratios always equals 1? b.C/T d.G/A 6. If a linear piece of DNA has three sites for a particular restriction enzyme, into how ma fragments will that restriction enzyme cut the DNA? a.2 b.4 c.3 d.5 e.The answer cannot be p 7-Assuming that most restriction sites are symmetrical about their midpoint (palindro of the following is most likely to represent a restriction site? B 5-GGATCC-3. ? 5-??????-3. E 5'-TTTGGG-3

Explanation / Answer

Question 1 : (B) cut the DNA fragment (gene) of interest.

Question 2 : (A) cutting by restriction enzymes "at the centre" in a specific nucleotide sequence

Question 3 : (A) moved from one organism to potentially any other organism.

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