My lab partner and I were unable to finish our lab, and do not have access to th
ID: 256648 • Letter: M
Question
My lab partner and I were unable to finish our lab, and do not have access to the microscopes necessary to do so. We are hoping to find answers to any questions unanswered in the picture, thank you! Procedure: Compound Microscope 1. With the diameter of the low power field (10x) the plastic millimeter rule on the sta microscope and focusing on it. What is the diameter of the field in millimeters? 2. Focus on one of your finger nails using the dissection microscope. With the ruler, measure the width in millimeters (mm) of your finger at the widest point of your nail. measure the ng ge 2. Convert this measurement to micrometers (um): 3. Focus on your thumb nail using the hiswectom microscope. With the ruler, measure the width in millimeters (mm) of your thumb at the widest point of your nail. 3. To calculate the diameter of high power field (40X) complete these two steps: a. divide the magnification of the high power field by the magnification of the low power field: b. then divide the diameter (of the low Review questions: powfiel (um) (CAnswer in question 2) by the quotient (3a) 1. What is the relationship between units of length, volume, and mass in using the metric system? Consider water and give equivalent measurements. This is the diameter, of the high power feld of your mieroscope in um Mass n d volum Should b nio-1) equveten. Is th suseu 4. Using a prepared slide of Paramecium, f ocus on 2. In estimating sizes of organisms, such as these organisms which are stained pi Estimate the length and width of one in micrometers (um) using the diameters of the microscopic fields calculated above. (Hint c. microscope, why are answers more accurate when using high power. Estimates will be more accurate on high puwer Length: Width 3. If your field of view is 2 mm with a 10X ective, what would be your field of view using a objective? Procedure: Dissection Microscope 4. If your field of view is 2 mm with a 10X obiective, what would be your field of view using a sox objective? 1. Using the dissection microscope measure the diameter of the field with the lowest magnification using a millimeter ruler. What the maximum diameter of the field of view in millimeters (mm)?Explanation / Answer
Procedure compound microscope:
4. Length of Paramecium: 195
Width of paramecium: 60
Procedure: Dissection microscope:
1. 24 mm
2. 7.5 mm
3. 10 mm
Review questions:
2. The accuracy increases due to higher resolution at higher magnification. (Definition of resolution: Ability to distinguish 2 adjacent point clearly.)
3. Field of viewnew = Field of viewold*Magnificationold/Magnificationnew
Field of view at 5X = 2 mm * 10X / 5X = 4 mm
4. Field of view at 50X = 2 mm * 10X / 50X = 0.4 mm
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