Time (seconds) H:0, by catalase activity (i.e. Figure 6 Pressure vs. Time The de
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Question
Time (seconds) H:0, by catalase activity (i.e. Figure 6 Pressure vs. Time The determination of the rate of decomposition of the slope) as a plot of Pressure vs. Time I the reaction with two drops of enzyme suspension, we may have to Important Depending on the rateo mange the number of drops of enzyme suspension needed for tubes 2-4 14. Con ine with test tubes 2 through 5 by following steps 1-13 using these new enzyme concentrations: add 5 drops of the enzyme solution to test tube 2, add 10 drops of the enzyme enzyme to the solution in Test Tube 5 (Why?) 15. Answer questions of Part A in the Report Sheet pages 33 and 34 as a group. Part B. The Effect of Temperature on an Enzymes' Reaction Rate Introduction The rate of a chemical reaction increases as temperature rises, in part because molecular velocity is increased (Brownian Motion). This means that substrate molecules collide more frequently with the active site of enzyme molecules. Generally, according to the Qso rule, a 10°C rise in temperature results in a two-fold increase in the rate of a chemical reaction. However, the activity of an enzyme is dependent upon the proper three-dimensional structure, and high temperatures may irreversibly denature an enzyme. The optimum temperature for enzyme activity, therefore, may vary depending on the structure of the enzyme, its source, and the nature of the substrate. The Set Up 1. Follow steps 1, 2, and 3 under Set Up for all experiments on page 26 to begin. 28 160 Laboratory Manual augh Revised Spring 2017Explanation / Answer
Hypothesis:
The enzyme activity should be highest at 37C. From 10C to 20C, the enzyme activity may increase. At 75C, the enzyme may denature.
Summary:
The effect of temperature on the enzyme activity is under investigation. Four test tubes, each containing equal volumes of 3% H2O2 and water, are kept at different temperatures (10C, 20C, 37C, and 75C). The substrates and enzyme solutions are equilibrated to the said temperature before mixing so that accurate temperature is maintained and no reaction occurs apart from that of the desired temperature. The activity of the enzyme is checked by measuring the rate of decomposition of hydrogen peroxide and representing it graphically.
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