o, seawater or other water, pickies 8.5 ra m L L of the Cated salts solution to
ID: 256451 • Letter: O
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o, seawater or other water, pickies 8.5 ra m L L of the Cated salts solution to some reasonable level: 15-25% is usually good. 3. Add carbon sources: 5 g/L peptone, 1 g/L yeast 4. If you want plates, add 1.5% agar. 5. Aut PROCEDURE: 2 solution. Carefully adjust the pH with concentrated Tris to be in the 7.5- extract, and 3 mL/L glycero 1. Dilute the concentrate 2. Add 5 mL/L of the CaCl2 8.5 range. usually pinkmight tr picil nocula, seawater or saline inland waters would be further information For plates, do NOT seal the oclave and inoculate. In addition to soil inocula, You may also try 'natural' salt rom bacteriorhodopsin-containing Halobacterium) inoculated onto n, and incubate in light. See The Prokaryotes or The Halo Handbook for (usually pink or purple, y pickles, salted meats, or sauerkraut. bacteriorhodopsin e plates with parafilm, as water will condense that will inhibit growth. -mediated ATP synthesis, incubate with direct light. 6. pa If you want to encourage bacteriorhodopsin ganisms that devel op, you can make a wet mount, but you will need to seal the ges with silicone or other sealant to avoid salt crystallization. To make a stain, some cells in 20% sterile brine, and spread a loopful on a slide. Air dry, then fix and coverslip ed y immersing in 2% acetic acid for 5 min. Remove and dry WITHOUT washing. Flood with ar violet for 3 min, then rinse and dry. For Gram staining, try fuchsin as the counter stain, ophiles are archaea that do not have peptidoglycan, and thus do but remember that many hal not Gram stain typically.Explanation / Answer
Please find the paragraph as below:
In order to prepare growth medium, sterile water of appropriate volume must be mixed with essential salts at a range of 15-25% while adding calcium chloride at a concentration of 5 ml/L of the solution at a pH range of 7.5-8.5 using tris base. Supplementation of this solution is performed with essential biomolecules such as peptone (5g/L), yeast extract (1 g/L) and glycerol (3 ml/L) and 1.5% agar (w/V) is dissolved for solidification of the media.
This suspension is dissolved carefully and autoclaved for sterilization. The sterile media is poured onto culture plates and different types of bacteria are grown using different types of selective salts. The microbes are allowed to grow for an appropriate period of time and visualized under microscope. The small amount of microbe is extracted from the culture plate and transferred onto a sterile coverslip duly sealed using silicone. A visualization stain is prepared by suspending a loop-full amount of microbes into 20% sterile brine, air dried and fixed and sequentially desalted in 2% acetic acid for 5 minutes. The slide is air-dried and washed in crystal violet for 3 minutes, rinsed in double distilled water and air dried. A counterstain is also prepared during the Gram's staining process using fuchsin but it must be carefully noted that the halophiles such as archaea are Gram-negative due to lack of peptidoglycans in their cell wall and do not give true negative Gram-staining results.
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