1. When you are inteneded to resolve very large DNA molecules on an agarose gel,
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Question
1. When you are inteneded to resolve very large DNA molecules on an agarose gel, needed
a. A low concentration gel
b. A high concentration gel
c. To digest the molecule first
2. Sodium Dodecyl Sulfate
a. Denaturing protein molecules
b. Resolving small size (60-400 bp) DNA molecule
c. Staining protein electrophoresis gel
d. Resolving large DNA or protein molecules
3. In a non-denaturing protein electrophoresis (native protein electrophoresis), the movement of protein in acrylamide gel depends on
a. Size only
b.Size, charge, and color
c. Size, shape, and net charge
d. Size and shape
Explanation / Answer
1. When you are inteneded to resolve very large DNA molecules on an agarose gel, needed:
a. A low concentration gel - because the large DNA molecules will not separate with enough resolution in high concentration gels.
2. Sodium Dodecyl Sulfate:
a. Denaturing protein molecules - SDS not only coats the protein molecule but also interacts with charges and linearizes the molecule thus conferring a uniform size-to-charge ratio.
3. In a non-denaturing protein electrophoresis (native protein electrophoresis), the movement of protein in acrylamide gel depends on:
c. Size, shape, and net charge - Net Charge will always be there as a function of charge-to-mass ratio. However, when native electrophoresis is carried out, we also obtain size and shape as potential factors of resolution.
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