1. There are two layers gel in a discontinuous gel electrophoresis method which

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Question

1. There are two layers gel in a discontinuous gel electrophoresis method which are characterized as:

a.Upper layer stacking gel with high gel concentration

b.Upper layer, stacking gel, lower concentration

c.Lower layer, resolving gel, higher concentration

d.A and B

e.B and C

2. In a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the movement of the protein molecules depends

a.Inversely on size of molecules

b.Directly on net charge of molecules

c.Inversely on shape of the molecules

d. Directly on size of the molecules

--In a discontinuous SDS-polyacrylamide gel, a sample of protein extract is loaded to the well and an electric current is applied.

3. What is the order of following molecules and ion from top to bottom in the resolving gel

a. Glycine, Cl-, Protein

b. Protein, Glycine, Cl-

c. Protein, Cl-, Glycine

d. Glycine, Protein, Cl-

4. What is the order of following molecules and ion from top to bottom in the stacking gel

a. Glycine, Protein, Cl-

b. Protein, Glycine, Cl-

c. Protein, Cl-, Glycine

d. Glycine, Cl-, Protein

5. We can use agarose gel as well as polyacrylamide gel to resolve both protein and DNA molecules

a. False, agarose gel is only used for DNA molecules and polyacrylamide is used for proteins

b. True, polyacrylamide gel is used for very large protein and DNA molecules

c. False, proteins cannot be resolved on an agarose gel

d. True, different concentrations of agarose and polyacrylamide can be used to resolve both molecules

1. E = both b and c

2. A = inversely on the size of the molecule

3. B = Protein , glycine and chloride

4. A = glycine , protein and chloride

5. D = DNA and protein both can be resolved using different concentrations of agarose and polyacrylamide

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