2 points Save Answer Question 4 E. coli was transformed with PGLO (shown below).

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2 points Save Answer Question 4 E. coli was transformed with PGLO (shown below). Transformants were cultured on two different media (Plate 1 and Plate 2). After overnight incubation at 35°C, the plates were photographed under a UV light. Explain why (be specifich the transformants on Plate 2 fluoresce green, while those on Plate 1 do not fluoresce. [2 pt; L2; II.B] pGLO Plate 1 Plate 2 LB/Ampicillin LB/Arabinose/Ampicillin TT TF Paragraph + Arial + 3 (12pt) + - E-T KDO S T T, ES T - - - fx Mashups - 1« © ® - SEE O IHTHLESS Qia -

Explanation / Answer

The plasmid pGLO has an ampicillin resistance gene (bla), a Green fluorescent protein (GFP) encoding gene and ara c gene which encodes for DNA binding protein Ara c. The plasmid also has pBAD promoter. In the presence of arabinose, the Ara c protein promotes the RNA polymerase binding to the promoter leading to the GFP transciption and then the translation to the GFP protein. However in the absence of arabinose, Ara c is not able to promote the binding leading to no expression of GFP.

Thus, in plate 1 there is no glowing as the arabinose is absent while in plant 2 transformants grow.

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