nsteans peosges Name The following structural genes and regulatory genes and ele

Question

nsteans peosges Name The following structural genes and regulatory genes and elements are important for the lac Review exercise operon: LacY, Lacz, Lact, Laco, LacP. Indicate on the diagram of the lac operon below, where these genes or elements are located. Lac operon where each What is the importance of each of these genes or regulatory elements? There are several mutations that may be observed in these genes and elements in bacteria. ( denotes normal wild type for that gene or element) 1. I denotes a defective repressor protein that does not bind the operator 2. makes the 3. O denotes a defective operator sequence that wont bind the repressor 4. Z denotes a gene producing a defective P-galactosidase enzyme repressor insensitive to lactose Normally, bacteria have one copy of their genome. They can become regions if they are supplied on an F factor plasmid. partially diploid for some For the following genotypes indicate if functional p-galactosidase will be produced when no lactose is present, just lactose is present, or both glucose and lactose are present. Genotype Lacls, Laco*, Lacz Lacl, Lacoc, Lacz Lacis, LacOC Lacz Lacl, LacO, Lacz Lacl, Lac0 LacS, Laco, LacZ: /F Lacl, Laco , Lacz Laci, Laco LacZ:/F Lact, Laco, Lacz Laci, LacOs LacZ/F Lact, LacO, Lacz Lacl, LacOC, Lacz'/F Lacls, LacO, LacZ

Explanation / Answer

genotype lactose no lactose glucose + lactose LacIs , LacO+, LacZ- - - - LacIs , LacOc, LacZ- - - - LacIs , LacOc, LacZ+ + - LacI- , LacOc, LacZ+ + - LacI- , LacO+, LacZ- - - - LacIs , LacO+, LacZ-/F LacI- , LacO+, LacZ+ + + + LacI+ , LacOc, LacZ-/F LacI- , LacO+, LacZ+ + - LacI+ , LacOc, LacZ+ / F LacI- , LacO+, LacZ- + - LacIs , LacOc, LacZ+/F LacIs , LacOc, LacZ- + + +

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