3- A researcher is performing indirect immunocytochemistry to assess myosin heav

Question

3- A researcher is performing indirect immunocytochemistry to assess myosin heavy chain expression in C2C12 cells. The researcher plans on using a primary antibody derived from rabbits and a secondary antibody derived from sheep. Which of the following blocking serums should the researcher use in this experiment.

a) Rabbit serum

b) Sheep serum

c) Goat serum

d) Mouse serum

e) Both c & d are correct

8- If the GLUT-4 transporter is at its saturation point, at what rate is it transporting glucose across the plasma membrane?

A) It is transporting glucose at its maximal rate, known as the Vmax

B) It is transporting glucose at its maximal rate, known as Km

C) It is transporting glucose at ½ of its Vmax

D) It is transporting glucose at ½ of its Km

E) The GLUT-4 transporter does not transport glucose, so the rate would be 0.

Explanation / Answer

(3) Answer; b) Sheep serum

Explanation: Principally we should use serum from the source species for the secondary antibody as opposed to the source species for the primary antibody. The serum carries antibodies which bind to reactive sites and prevent the nonspecific binding of the secondary antibodies

(8)A) It is transporting glucose at its maximal rate, known as the Vmax

Vmax "represents the maximum rate achieved by the enzyme/transporter, at saturating substrate concentrations"

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