5) (2 PTS) You are studying DNA replication. You isolate all the replication enz

Question

5) (2 PTS) You are studying DNA replication. You isolate all the replication enzymes from bacteria, and try to replicate your DNA in a test tube (in vitro). You mix your enzymes and template with pure dNTPs and sit back and wait, but nothing happens. What have you forgotten to add? (Note: All of your enzymes are functional, and the salt and the pH levels are normal) 6 (4 PTS)There are several aspects of DNA polymerases that tell you about their probable functions in the cell. These are processivity, 3'à5' exonuclease activity, requirements for viability, 5à3' exonuclease activity. Describe what each of these mean and indicate what they tell you about the polymerase

Explanation / Answer

5) Everything is there in the tube except the primers.

Primers are needed to initiate the replication process as the bacterial dna polymerase is RNA dependent dna polymerase and need primer to start the replication.

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