5. Define the types of repetitive sequences of DNA that are depicted in the foll

Question

5. Define the types of repetitive sequences of DNA that are depicted in the following diagram, then, for each genetic marker at the bottom, give an example of how it is used in recombinant DNA technology. Note: write your answers in DNA Highly repetitive Middle Satellite DNA nterspersed repeats Types of repetitive sequences of DNA Multiple SINES LINEs copy genessatellites satellites Examples of genetic markersRNA genes VNTRS STRs Alu L1 Figure 12-14 from Klug, WS, Cummings, M.R., Spencer, C.A, Palladino, M.A, and Killin, DJ. 2019 Concepts of Genetics 12th Edition Pearson Education,Inc.)

Explanation / Answer

Answer : Multi copy gene are that gene which is available in multiple copies a single gene.

Mini satellite : These are repititive DNA in which a certain DNA sequence around 10-60bp is repeated 5-50 times.

Micro satellite: These are repititive DNA in which a certain DNA sequence around 1-6bp is repeated 5-50 times.

SINEs: Short Interspersed Nuclear Elements are the non coding DNA about 100-700bp which are present in high amount in a genome.

LINEs: Long interspersed nuclear elements are group of retrotransposons, which is spread throught the genome. they are around 7000 bp long.

Uses of genetic markers

rRNA gene (rRNA 16s for prokaryotes and 18s eukaryotes ) is markers used in taxonomy to identify evolutionary relationship.

VNTRs= variable number tandem repeats uses in genetic Analyis to identifying the person. Fingerprinting technique use the VNTRs.

STRs,= short tandem repeats are used in comparing specific loci on DNA from two or more samples.

Alu = it's variation are used to study the movement and ancestory of human population.

L1= L1 sequences in technique which is used to identify L1 insertion In genomic DNA in any sample.

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