You started work in a transcription lab working a with a novel bacteria phage. Y

Question

You started work in a transcription lab working a with a novel bacteria phage. You isolated an enzyme that has RNA polymerase activity, and you discovered that your new viral RNA polymerase has a unique property. Unlike E. coli RNA polymerase, which prefers starting transcription with ATP, your new viral polymerase prefers starting transcription with GMP. You know the directionality of RNA chain growth (3’-to-5’ or 5-to-3’) by your new RNAP.

A. Explain in what direction (3’-to-5’ or 5-to-3’) this enzyme synthesizes RNA and how you know this.

B. What other simple experiment can you do to show that your conclusion is correct, provided you have full access to all rNTPs, 3’-dNTPs, 2’-dNTPs?

Explanation / Answer

The direction of synthesis of RNA strand takes place of the 5' to 3' direction.The template strand of dsDNA is the strand whose RNA is made.

B)The rNTPs is required for the synthesis of RNA strand.The unwinding of DNA strand leads to unwind,rewind .

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