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ID: 255590 • Letter: Q
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Questions with asterisks are available for presentations Volunteers will be chosen day of class A glycine residue is in position 210 of the tryptophan synthetase enzyme of wild- type E. coli If the codon specifying glycine is GGA, how many single-base substitutions will result in an amino acid substitution at position 210? Which amino acids could result from a single-base substitution in GGA (gly)? Indicate the base change(s) that produces each new amino acid 2Consider the following segment of DNA, and assume that the top strand is the template strand used by RNA polymerase.] 5' GCT TCC CAA 3 3, CGA AGG GTT 5 Draw the RNA transcribed (Label its 5' and 3 ends) Draw the corresponding amino acid chain (Label its amino and carboxyl ends) a"Consider the following sequences Wild Type Mutant:. 5' AAUCCUUACGGA 3'... 5' AAUCCUACGGA 3 What type of mutation is depicted by these mRNA sequences? How would this change the amino acid sequence produced?Explanation / Answer
Only one single base substitution is required.
As, glycine is coded by the codon” GGA”, is we substituted middle G with C, then it became alanine amino acid. And, second possibility is this when middle G is substituted with A, it become “glutamic acid”.
DNA TEMPLATE: 5’ GCTTCCCAA3’
RNA transcribed: CGAAGGGUU
Corresponding amino acid: Arginine-Arginine-valine
It is a type of deletion mutation (frame shift mutation) as one base is deleted in the mutant strand.
The amino acid sequence will remain same as that of wild type .
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