1.) A single-pass transmembrane protein is destined to be transported to the pla

Question

1.) A single-pass transmembrane protein is destined to be transported to the plasma membrane. In the wild-type protein, a lysine flanks the transmembrane domain on the N-terminal side, and a glutamic acid flanks on the C-terminal side. Both the N- & C-terminal soluble domains carry sequences that can be glycosylated by oligosaccharyltransferase.

A.) After the protein is transported to the plasma membrane, will the N- or the C-terminal soluble domain be on the extracellular side?

B.) Is the glycosylation added in the ER onto the N- or the C- terminal soluble domain?

Explanation / Answer

1a. Generally C terminal soluble domain will be in extra-cellular space because most of surface protein carries a negative charge on it. And C terminal contains a glutamic acid residues that can easily converted to negative charge -COO- .

B. Glycosylation will be ocuur on C terminal soluble domain.

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.