Morty thinks that he can purify a protein better than his grandfather, Rick. Pro

Question

Morty thinks that he can purify a protein better than his grandfather, Rick. Prove mathematically that Morty is wrong by completing the purification table below (show all work, 4.15 points): Morty's Purification Crude Salt Precipitation Size Exclusion on Exchange Specific Activit Fold Purification Enzyme units 8400 7982 7574 5898 Percent Yield Protein 1022 362 135.2 7.6 Fold Purification Enzyme units 11760 11168 10348 Specific Activi Rick's Purification Crude Salt Precipitation on Exchange Protein Percent Yield 1428 506 10.2

Explanation / Answer

Answer:

Specific activity can be determined as:

Fold purification can be calculated as:

Percent yield can be obtained as:

Applying above formula, we get:

Based on the data analysis, the fold purification obtained after final step for Rick' protocol is 123.2 and Percent yield is 88% whereas for Morty's protocol fold purification obtained after final step is 94.4 and Percent yield is 70.2%.

So Rick's method results in higher fold purification and more percent yield as compared to Morty's method.

Morty's Purification Protein (mg) Enzyme (Units) Specific Activity Fold Purification Percent Yield Crude 1022 8400 8.2 1.0 100.0 Salt Precipitation 362 7982 22.0 2.7 95.0 Size Exclusion 135.2 7574 56.0 6.8 90.2 Ion Exchange 7.6 5898 776.1 94.4 70.2

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