Use the biochemical pathway below to answer this question. Strain 1 and Strain 2
ID: 254375 • Letter: U
Question
Use the biochemical pathway below to answer this question. Strain 1 and Strain 2 cannot grow on minimal media and are defective at the enzymatic steps indicated on the pathway. Fill in the blanks with + if the strain will be able to grow on minimal media and the intermediate specified or - if the strain cannot grow on minimal media with the intermediate provided.
F
Question 2
You are given the biochemical pathway below. Seven mutant strains (labeled S1 - S7) are defective in this pathway and cannot produce the end product when provided with minimal media. Each mutant strain is defective in only the one step indicated by the path. Select all metabolites that when added to minimal media (one at a time) will allow the mutant strain S4 to produce the end product in the reaction. If none of these metabolites will rescue the mutant strain, select "None of These".
Select one or more:
C
G
None of These
E
D
M
Strain 1 Strain 2 A ? B ? C ? D ? E ?F
Explanation / Answer
Answer 1:
According to biochemical pathway, strain 1 is unable to convert reactant A to its product B, but it has functional pathway downstream of B. it means that strain 1 will not survive unless it is provided with an intermediate B or the other intermediates in the pathway that comes after B.
likewise, strain 2 is unable to convert its reactant C to its product D but it has functional pathway downstream of D. it means that strain 1 will not survive unless it is provided with an intermediate B or the other intermediates in the pathway that comes after B. so the table would be:
Intermediate
Strain 1
Strain 2
A
-
-
B
+
-
C
+
-
D
+
+
E
+
+
F
+
+
Intermediate
Strain 1
Strain 2
A
-
-
B
+
-
C
+
-
D
+
+
E
+
+
F
+
+
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