18. You have a bacterial culture solution. When you directly measured the OD6oo,

Question

18. You have a bacterial culture solution. When you directly measured the OD6oo, of the culture solution, it was 3. Then, you performed a 10-fold dilution and measured the ODoo of the diluted solution, which was 0.5. Thirdly, you performed another 10-fold dilution and measured the OD6c of the diluted solution which was 0.07. Assuming that OD6o1 is equivalent to 10 cells/ml, how many cells do you have in 1 ml of the original culture? A)3 x 10 cells B) 6 x 10 cells C)7 x 10 cells D) 8x 10 cells E) 3 x 1010 cells

Explanation / Answer

How many cells do you have in 1 ml of original culture?

C) 7 X 109 cells

The OD600 usually depends on the size of the cell.

OD600 of 1.0 = 109 cells/ml.

After 102 fold dilution, we got OD600 = 0.07.

So, the number of bacterial cells present after 102 fold dilutions is 0.07 X 109.

Therefore, in 1 ml of original culture, the number of cells = 0.07 X 109 X 102 = 7 X 109.

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