NAME: 5\' ttggtggtga ggecetggge aggetgetgg tggtctaccc ttggacecag 3\'aacetgggtcs
ID: 253886 • Letter: N
Question
NAME: 5' ttggtggtga ggecetggge aggetgetgg tggtctaccc ttggacecag 3'aacetgggtcs B1. Please consider the sequence shown above. A sequencing reaction is carried out on this molecule. DNA polymerase is moving right to left on the molecule shown in this reaction, ddA's are blue, ddT's are green, ddC's are black and doG's are red. Please answer the following about this situation. Draw the first ten peaks that will be seen on the sequencing trace, beginning at the smallest a. molecule and going toward the larger ones. State the color of each peak (You can draw bands, if you prefer, but be sure to say which is which and what color they are.) Another reaction was done using the same template as above. But this time, the graduate student doing the reaction put in only ONE dideoxyribonucleotide triphosphase, ddATP. The rest of the nucleotides were all normal. Please draw the appearance of the peaks (or bands) that will be observed into your picture above, next to or below the first reaction. b.Explanation / Answer
Q. A The colour pattern for 10 nucleotides will be RED-RED-RED-GREEN-BLUE-GREEN-BLUE-BLACK-BLACK-BLUE
Q. B. so now only one coloured nucleotide is used which is adenosine so other will not going to give colour peak. so only blue will comes as in bold.
RED-RED-RED-GREEN-BLUE-GREEN-BLUE-BLACK-BLACK-BLUE.
if the questioner mean all are coloured by saying all other are normal then only blue left and other will gives colour peak as
RED-RED-RED-GREEN-BLUE-GREEN-BLUE-BLACK-BLACK-BLUE
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