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w History Bookmarks Window Help com Order the step reired to sraiye gore BLY 302 - Spring18-KROETZ: Homework 9 ng Jump to... Sapling Learning Tissue-spedific microarray analysis facilitates the comparison of gene expression in different tissues. in order to prepare a sample for microarray analysis issue, reverse transcribed to generate complementary DNA (cONA). RNA (mRNA) is extracted from a partoular and labeled with a fluorescent probe. n stomach and intestinal tissues. The cDNA The microarray below shows comparison of gene expression in from stomach tissue was labeled with red fluorescent probes, whereas the cDNA from the intestinal tissue was labeled with green fuorescent probes Gene A s primanily expressed only in stomach issuewhereas expressed in intestinal tissues, Genes C and D are responsible for basic cellular B is primarily ctions and are equally expressed in stomach and intestinal tissues. information provided. If there Match the spots on the microarray with the appropriate genes based on the are multiple possible answers for a particular gene, choose any of the possible options Genes O Previous 9 Check Answer 0 Next V Hint about us careers privacy polley tems of use contactus heipExplanation / Answer
DNA microarray can be used to analyse tissue specific expression of thousands of gene using the fluorescent probe. The results can be obtained as tiny spots.
Keeping this information in mind we can interpret the results in the following manner,
cDNA expressed in the intestinal tissues are labelled with green fluorescent probe.
A GREEN dot at first row, second column represents GENE B expressed in the intestinal tissues.
cDNA expressed in the stomach tissues are labelled with red fluorescent probe.
A RED dot at second row, first column represents GENE A expressed in the intestinal tissues.
There are some gene namely p53 gene can be found expressing in all the tissue, so both the probe emit fluorescent. Red + Green light gives out yellow colour light. This is an indication that this particular cDNA is expressed in those two tissue.
A YELLOW dot at first row, third column can be represented as GENE C and the other YELLOW dot at third row, second column can be represented as GENE D.
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